Engineering of Microbial Biosynthetic Pathways

This book provides a comprehensive overview of the basic and advanced metabolic engineering technologies used to generate natural metabolites and industrially important biomolecules.

Metabolic engineering has the potential to produce large quantities of valuable biomolecules in a renewable and sustainable manner by extending or modifying biosynthetic pathways in a wide range of organisms. It has been successfully used to produce chemicals, drugs, enzymes, amino acids, antibiotics, biofuels, and industrially important pharmaceuticals.

The book comprehensively reviews the various metabolites detection, extraction and biosensors and the metabolic engineering of microbial strains for the production of industrially useful enzymes, proteins, organic acids, vitamins and antibiotics, therapeutics, chemicals, and biofuels. It also discusses various genetic engineering and synthetic biology tools for metabolic engineering. In closing, the book discusses ethical, patenting and regulatory issues in the metabolic engineering of microbes. This book is a valuable source not only for beginners in metabolic engineering, but also students, researchers, biotechnology and metabolic engineering based company.

Reviews basic and advanced tools for the metabolic engineering of microbial strains to generate industrially important bioproducts

Presents recent advances in metabolic engineering, bioreactors, bioprocesses, strain development, pathway design, construction and optimization

Describes essential synthetic biology tools, including CRISPR-Cas systems for targeted genome editing in metabolic engineering

Addresses the mathematical modeling of metabolic pathways

Highlights the ethical and regulatory challenges posed by the metabolic engineering of microbial strains